This experiment utilized our 24-well plates, which have two flow channels that merge into a common flow cell. This creates two distinct environments with a diffusional boundary in the middle, allowing for unique chemotaxis and migration assays under shear stress. Conditions can be varied by fluid type, gas composition, pH, etc. The paper can be accessed here: https://www.pnas.org/doi/10.1073/pnas.1600760113.