Leukocyte Adhesion Cascade

Study all steps of the leukocyte adhesion cascade with one system

The leukocyte adhesion cascade consists of capture, rolling, slow rolling, arrest, strengthening/spreading, and transmigration. Typically, analyses of these steps require at least 3 separate systems and/or assays.

BioFlux shear flow systems enable the visualization and analysis of all of the steps of the leukocyte adhesion cascade under physiological/pathological shear flow using one system.

General BioFlux workflow for cell adhesion cascade assays

Key BioFlux Advantages For Cell Adhesion Cascade Studies

Image and quantify live cell adhesion and rolling in a physiological environment using primary endothelial cells, human peripheral blood mononuclear cells, and more
Utilize time-lapse microscopy and powerful post-experiment image analysis software to determine rolling distance under flow
Examine up tp 24 different monolayers at one time to determine IC₅₀ concentrations or screen multiple compounds
Study truly biological cell adhesion and transmigration in the same assay

Leukocyte adhesion cascade assays using BioFlux

Adhesion

Rolling

Transmigration

Recombinant human vimentin binds to P-selectin and blocks neutrophil capture and rolling on platelets and endothelium

The top channel shows the inhibition of neutrophil adhesion by recombinant human vimentin. The bottom channel shows neutrophil adhesion under control conditions.

The vimentin rod domain blocks P-selectin-P-selectin glycoprotein ligand 1 interactions to attenuate leukocyte adhesion to inflamed endothelium

Neutrophil rolling on endothelial cells under shear flow. Scale bar = 50 µm

Ezrin and Moesin Are Required for Efficient T Cell Adhesion and Homing to Lymphoid Organs

Tracking of the transmigration of CMTMR stained wild-type T cells (red) and CFSE stained ERM-deficient T cells (green) through TNF-α treated 3B-11 endothelial cells.