Antimicrobial Screening
Visualize & Quantify Antimicrobial Investigations
Antimicrobial screening often requires the assessment of many compounds under near identical conditions. This makes the use of microplates popular, however, biofilms grown under static conditions are often malnourished and underdeveloped. This can lead to false positive results.
BioFlux shear flow systems can accommodate up to 24 experiments in parallel, under biologically relevant conditions. This enables the acceleration of antimicrobial discoveries by reducing false positives without sacrificing throughput. Â Â
BioFlux antimicrobial screening workflow
Antimicrobial investigations using BioFlux
(A) S. mutans biofilms were cultured in a BioFlux system for 18 to 24 hours, inoculated with different antibacterial agents, and stained (live cells stained green and dead cells stained red). (B) These results were graphed, showing statistically significant differences between the control and the antimicrobial-treated groups.
Inhibition of biofilm development of the MRSA strain USA300 FPR3757 by tilmicosin under a flowing condition (BioFlux 1000 system). The 16 h biofilm formed in the channels was photographed (Left panel). The amount of biomass was monitored and analyzed with BioFlux Montage software 2.3 in real time (Right panel).
Poster spotlight: Growing Mature Biofilms for Antimicrobial Screening, Host-pathogen Interactions, and Adhesion
Key BioFlux Advantages For Antimicrobial Screening Studies
- Analyze the effectiveness of different antimicrobial compounds by growing biofilms under controlled shear flow, mimicking the conditions of natural growth
- Use the all silicone plate format for antimicrobial compounds relating to catheter-associated infections
- Custom substrate plates feature the ability to add any material to the bottom of microfluidic flow chambers, and enable a full range of experimental protocols for a variety of microbes and antimicrobial compounds
- Discover new antimicrobial compounds and help eradicate antibiotic tolerance and susceptibility with a flow-based system
Maximize Screening Throughput with BioFlux 1000HT
- Customizable microscope
- Automated stageÂ
- Compatibility with 6, 24, and 48-well BioFlux plates
- Dual gas & Optional temperature control
- Montage Plus acquisition and analysis software
Automated image acquisition using a BioFlux 1000zÂ
Article Highlight
In Silico Screening and In Vitro Assessment of Natural Products with Anti-Virulence Activity against Helicobacter pylori
Helicobacter pylori is one of the most frequently encountered human pathogens. Infection with H. pylori can lead to the development of various gastric diseases, such as peptic ulcers and gastric cancers.Â
In this article, Spiegel et al. virtually screened 791 natural substances for anti-biofilm activity. To find the most effective antimicrobial substance, the group leveraged the high throughput capacity of the BioFlux 1000Z to test the 10 top candidates under physiological flow and no flow environments.
