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Salmonella enterica serovar Typhimurium (Salmonella Typhimurium, S .tm) induces self-limiting gastroenteritis in humans and systemic infection in mice—a commonly used typhoid fever model. In this study, investigators used a CASY cell counter and analyzer to count and assess the viability of S.tm and macrophages. The macrophages that were counted using CASY were then assessed for the production of reactive oxygen species (ROS) in response to bacterial infection. The investigators leveraged CASY to develop a protocol to measure ROS in macrophages.