Genomics
Connect Phenotype to Genotype
The CellRaftA microscale polystyrene growth surface within an array used to spatially segregate cells/colonies while maintaining shared media access, enabling imaging over time and targeted isolation. More AIR System’s unique design allows users to connect downstream nucleic and protein profiles to a specific individual cell, providing greater value to each experiment.
Know Exactly What Cells Are in Your Library
With typical genomic experiments, even an enriched cell population can contain undesired cells, dead cells, doublets, debris, or other artifacts. The imaging capabilities of the CellRaft AIR SystemAn all-in-one platform for imaging, identifying, and isolating viable single cells, colonies, or organoids using CellRaft Arrays and software-guided selection with CellRaft Cytometry. More provide the user with the ability to identify single cells and use software-guided tools to select desired cells based on viability or phenotypic criteria immediately after visualization.
Fast, effective and gentle automated isolation helps maintain native expression profiles. Selected cells are moved into lysis buffer quickly and gently with no need for trypsin, so phenotypes remain unperturbed, and quality remains high. No minimum required input and gentle movement allows the use of many cell types.
- Track thousands of cells simultaneously in one consumable
- Visualize cell stages and mutations
- Identify biomarkers using live staining
- Reduce the chances of doublets compared to fluorescence-activated nuclear sorting
- Improve the quality of sensitive samples
Accelerating Generation of Single Cell Clones
[glossary]A Comparison of Technologies[/glossary]
Functional Genomics
Pooled image-base screening of mitochondria with microraft isolation distinguishes pathogenic mitofusin 2 mutations
This article presents a generalizable functional genomics platform combining high-content imaging, machine learning, and CellRaftA microscale polystyrene growth surface within an array used to spatially segregate cells/colonies while maintaining shared media access, enabling imaging over time and targeted isolation. More isolation termed “Raft-Seq.” This user isolates CellRafts containing cells with unique phenotypic differences allowing genotype and phenotype to be linked. In this study, Mitofusion 2 mutations were able to be distinguished efficiently.
Article quotes about CellRaft Technology
- “Associates a sequence with the specific image of cell(s)”
- “Capture individual live cells for growth alongside their original microscopy data is unique among pooled screening platforms”
Transcriptomics and scRNA-seq
Single-Cell Identification of Melanoma Biomarkers in Circulating Tumor Cells
This article presents a new approach to isolate circulating tumor cells (CTCs) from a more heterogenous population using the CellRaft ArrayConsumables containing thousands of microwells (containing CellRafts) for spatial segregation, imaging, and isolation of single cells, monoclonal colonies, or 3D cultures. More for used with both patient and cultured cells. Using a broader antibody cocktail, CTCs were first live-stained using direct immunofluorescence, seeded on an array, and then isolated for single-cell RNA analysis. Using the CellRaft ArrayConsumables containing thousands of microwells (containing CellRafts) for spatial segregation, imaging, and isolation of single cells, monoclonal colonies, or 3D cultures. More only specific melanoma CTCs were isolated from the surrounding population demonstrating accuracy and precision of this technology.
Article quotes about CellRaft Technology
- “Various genetic biomarkers can be identified and tracked to aid in clinical treatment”
- “Can identify disease specific genetic markers through live-staining”
- “Can be paired with a wide variety of downstream analysis like single-cell CHIP, ATAC sequencing and exome sequencing, and culturing/outgrowing viable cells”
Whole-genome Amplification
Single-cell sequencing of the small and AT-skewed genome of malaria parasites
This article focused on understanding more about a life-threatening disease, malaria, which is caused from specific parasites. Using the CellRaft ArrayConsumables containing thousands of microwells (containing CellRafts) for spatial segregation, imaging, and isolation of single cells, monoclonal colonies, or 3D cultures. More, this user was able to scan the Array on the CellRaft AIR SystemAn all-in-one platform for imaging, identifying, and isolating viable single cells, colonies, or organoids using CellRaft Arrays and software-guided selection with CellRaft Cytometry. More to identify stage specific phenotypes of single cells infected with parasites using SYBR green and Mitotracker Red staining to visualize parasite DNA and mitochondrion. This visualization allowed for the user to identify CellRafts of interest and isolate specific ones containing single infected cells for whole genome amplification later identifying SNPs and CNVs.
Article quotes about CellRaft Technology
- “requires low capture volume (minimum 2uL)”
- “Visual confirmation of parasite stages”
Single Nucleus Sequencing
Analyzing somatic mutations by single-cell whole-genome sequencing
This user has expanded their single-cell isolation from originally using only adherent fibroblast cells to now other cell types including lymphocytes, hepatocytes, and nuclei for single cell isolation and sequencing. This flexibility demonstrates CellRaftA microscale polystyrene growth surface within an array used to spatially segregate cells/colonies while maintaining shared media access, enabling imaging over time and targeted isolation. More Technology’s ability to be compatible with diverse cell types. Additionally, using the AIR System allowed for visualization of cells prior to sequencing while avoiding substantial damage involved in previous work with FACS.
Article quotes about CellRaft Technology
- “Can better observe and transfer cells without substantial damage”
- “Avoid harmful conditions when isolating single cells or nuclei”
- “Array is composed of tens of thousands of micro rafts that can be released from the bulk array and transferred to a PCR tube or plate”
Copy Number Variation
Investigation of somatic CNVs in brains of synucleinopathy cases using targeted SNCA analysis and single cell sequencing
This journal focused on isolating nuclei using a CellRaft ArrayConsumables containing thousands of microwells (containing CellRafts) for spatial segregation, imaging, and isolation of single cells, monoclonal colonies, or 3D cultures. More to evaluate somatic SNCA CNV differences for studying neurodegenerative disorders like Parkinson’s disease and multiple system atrophy. Following protocols for adhering nuclei to the Array, images were taken to obtain visual evidence of CNVs among different nuclei after whole genome sequencing.
Article quotes about CellRaft Technology
- “Allows visual review”
- “Reduces the chance of doublets compared to fluorescence-activated nuclear sorting (FANS)”
- “Images can be correlated with the individual cell WGS results”
Functional Genomics
Accurate identification of single-nucleotide variants in whole-genome-amplified single cells
This journal highlighted the flexibility of CellRaftA microscale polystyrene growth surface within an array used to spatially segregate cells/colonies while maintaining shared media access, enabling imaging over time and targeted isolation. More Technology for multiple applications. In this article, the user isolated single cells as well as monoclonal colonies using the same instrument to evaluate SNVs in both single cells and clonal colonies. With the flexibility of the AIR System, users could isolate cells in both PCR tubes and 96-well plates for their downstream assay of choice. CellRaftA microscale polystyrene growth surface within an array used to spatially segregate cells/colonies while maintaining shared media access, enabling imaging over time and targeted isolation. More Technology aided this researcher in demonstrating a better solution for genome-wide DNA mutation analysis in single cells.
Article quotes about CellRaft Technology
- “Validated no contamination from cell-free DNA in WGS”
- “Traceable locations of cells”
Accelerate the Path to Discovery
How It Works
Image Cells
[glossary]Cells are plated on a CellRaft® Array using standard plating techniques, eliminating the need for trypsin, scraping, high-pressure fluidics, and limiting dilution. The array is scanned, imaged, and recorded, allowing the user to track cells as they grow over time. [/glossary]
Grow Cells
[glossary]Target cells grow unperturbed in optimized conditions in the imaging platform, increasing cell viability, and downstream read depth. The CellRaft Arrays allow different media, compound dosing, and growth conditions to be tested on a single consumablepropriety culture dish. [/glossary]
Isolate Cells
[glossary]When target cells are ready for collection, the gentle release mechanism leaves cells unperturbed, so transfer to a PCR or culture plate won’t affect the quality of the cells for downstream analysis. Cells are also moved into lysis buffer quickly, preventing the degradation of genetic material in sensitive cell lines.[/glossary]
