To elucidate the mechanisms driving chemoresistance in Ewing’s sarcoma patients, researchers at the University of North Carolina at Chapel Hill exposed Ewing’s sarcoma cell lines, MHH-ES-1 and TC-71, to common chemotherapeutics SN-38 and etoposide to select for a chemoresistant population of cells. These populations were then plated using limiting dilutionA statistical cloning approach where cells are diluted to low density to “hopefully” seed one cell per well; commonly used but can be low-efficiency for difficult-to-clone lines. More to generate a monoclonal population, but single cells failed to expand. The researchers turned to Cell Microsystems to leverage the CellRaftA microscale polystyrene growth surface within an array used to spatially segregate cells/colonies while maintaining shared media access, enabling imaging over time and targeted isolation. More Technology, a novel single cell cloning and cell line development platform.
