No More Limiting Dilution

Single cells are grown in a flask-like environment, without physically separating them, eliminating perturbation to cell physiology and ensuring the viability and vitality of single cells as they develop into clones. The CellRaftA microscale polystyrene growth surface within an array used to spatially segregate cells/colonies while maintaining shared media access, enabling imaging over time and targeted isolation. More AIR® system is able to scan more than 40,000 individual cells per CellRaft ArrayConsumables containing thousands of microwells (containing CellRafts) for spatial segregation, imaging, and isolation of single cells, monoclonal colonies, or 3D cultures. More, which is 500X more cells than a traditional 96-well plate with limiting dilutionA statistical cloning approach where cells are diluted to low density to “hopefully” seed one cell per well; commonly used but can be low-efficiency for difficult-to-clone lines. More.