Resources

Have you ever wondered why your monoclonal colonies do not survive? This white paper gives an overview of current methods for creating monoclonal cell lines (including limiting dilution, single cell dispensing, flow cytometry, and cell sorting) and discusses the challenges and how to overcome them. Valuable tables compare technologies, price, and scientific workflow as well as the advantages and disadvantages of each method. Read this white paper to achieve: Thorough review of technology comparisons of different methods, costs associated with different technology, workflow, and sample requirements. Clear understanding of the impact of using cell sorting and dispensing in cell viability and cell health impacting outgrowth efficiency and number of clones produced. Recognition of technologies that allow for flask-like culture conditions to identify, select, isolate, and nurture highly viable clones with outgrowth efficiency above 90%.