Platelet Aggregation & Adhesion
Hematology with BioFlux
Traditional platelet aggregation assays, such as light transmission and multiple-electrode aggregometry, rely on aggregation agonists in static conditions, thereby limiting their physiological relevance. In addition, these assays lack the ability to examine cell-cell interactions, platelet adhesion to various substrates, and modeling platelet function in various areas of the vasculature by assaying under multiple shear stresses.
BioFlux shear flow systems overcome these disadvantages by enabling the visualization and analysis of platelet aggregation and adhesion under physiological and pathophysiological shear stresses. High-throughput microplates, combined with versatile sample capabilities including whole blood can reduce assay time to 3 hours, making BioFlux the ideal system to investigate platelet function in disorders such as COVID-19 and Sickle cell disease. Â Â Â Â
One system, many assays
BioFlux hematology assays:
- Platelet rolling
- Platelet adhesion & adhesion inhibition
- Platelet aggregation Â
- Thrombus formation & reversibility
- Drug discovery & development
- Assessment under variable flow rates
- Analysis under pulsatile flow
Key BioFlux Advantages For Platelet Aggregation & Adhesion Studies
- Physiological assessment of adhesion and aggregation using primary endothelial cells, human peripheral blood mononuclear cells, and more
- Assay platelet adhesion and aggregation kinetics in about 3 hours
- Utilize time-lapse microscopy and post-experiment image analysis to determine rolling distance under flow
- Examine up to 24 different monolayers on one plate to determine IC50 or screen multiple compounds under identical conditions
- Calculate area of platelet adhesion by utilizing Montage Plus analysis capabilities
BioFlux platelet aggregation & adhesion assay general workflow
Hematology assays using BioFlux
Efficacy of platelet-inspired hemostatic nanoparticles on bleeding in von Willebrand disease murine models
Imaging of calcien (green) labeled human platelet adherence and aggregation with rhodamine-B labeled (red) synthetic platelet nanoparticles.Â
Platelet adhesion and aggregate formation controlled by immobilised and soluble VWF
Aggregate formation from whole blood being perfused over von Willebrand factor using an increasing shear rate followed by cessation of flow. Â
Application note: Platelet adhesion and aggregation assay
Download this application note to learn how to conduct a platelet adhesion and aggregation assay using a BioFlux shear flow system.Â
Key Highlights:
- Assessment of platelet adherence to multiple substrates in the same experimentÂ
- High-throughput pharmacological screeningÂ
- Simple replication of in vivo platelet-vascular interactions
