Stem Cells
Are iPSCs a Bottleneck in Your Lab?
Generate 100s of healthy monoclonal iPSCs or hESCs
- Increase clonal iPSC generation by 25X compared to limiting dilution
- Track and trace iPSCs from single cell to clone
- Expand, characterize, edit, and reprogram
- Confirm pluripotency on-array prior to clonal expansion
- Reduce time, consumables, materials, and labor required for iPSC clonal development
- Enable 2D and 3D applications including cell line development, reprogramming, and differentiation
- Fully automate the gentle isolation of viable, validated iPSC cell lines
Accelerating The Use Of Patient-derived hiPSC Neuronal Models By Increasing Efficiency Of Reprogramming And differentiation Using Cellraft Technology
Increase in iPSC Monoclonal Colony Generation and Outgrowth
Track and Trace from Single Cell to Clone
Enable All iPSC Workflows Using CellRaft Technology
- Expand the clonal population on the array
- Characterize for phenotyping or pluripotency
- Differentiate into 3D tissues or organoids
- Reprogram differentiated fibroblasts into pluripotent stem cells
Reduce Time, Consumables, Materials, and Labor
We compared clonal iPSC development using CellRaftA microscale polystyrene growth surface within an array used to spatially segregate cells/colonies while maintaining shared media access, enabling imaging over time and targeted isolation. More Technology to traditional limiting dilutionA statistical cloning approach where cells are diluted to low density to “hopefully” seed one cell per well; commonly used but can be low-efficiency for difficult-to-clone lines. More. Using the CellRaft ArrayConsumables containing thousands of microwells (containing CellRafts) for spatial segregation, imaging, and isolation of single cells, monoclonal colonies, or 3D cultures. More, we were able to generate over 200 single cell-derived iPSC clones on a single array, compared to 10 clones in a single 96-well plate with limiting dilutionA statistical cloning approach where cells are diluted to low density to “hopefully” seed one cell per well; commonly used but can be low-efficiency for difficult-to-clone lines. More. This workflow required 1000X less iPSC coating and 2000X less media per cell screened.
Grow and Isolate iPSC-derived Organoids
CellRaftA microscale polystyrene growth surface within an array used to spatially segregate cells/colonies while maintaining shared media access, enabling imaging over time and targeted isolation. More Technology can be used to grow and maintain hundreds of individual organoids. You can serially image the same organoid over time and phenotypically characterize organoids to identify the ones of interest.
