Shear Flow

BioFlux Systems

In Vivo Biology Isn't Static. Why Are Your Assays?

BioFlux enables you with the power to introduce physiological and pathological shear flow to your research and drug discovery experiments, effectively emulating in vivo conditions and revealing the true biology.

Bridge the gap between in vitro and in vivo experiments with controlled shear stress and complete microenviromental control
Achieve high throughput imaging of up to 24 experiments per plate by growing, assaying, and imaging in a well plate
Easily acquire high-resolution images with coverslip glass bottom plates
Customize assays to fit your needs with dual flow microfluidic channels and pulsatile flow with wall shear stresses from 0-200 dyn/cm²
Easy-to-use and cost-effective - Calculate Your Savings

How BioFlux Works

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The Next Generation of BioFlux Shear Flow Systems

The new BioFlux 200+ and BioFlux 1000HT enhance the production of physiologically accurate and reproducible data by including pulsatile flow and dual gas capabilities, at a better price than before.

BioFlux 200+
The BioFlux Solution for Your Existing Microscope

BioFlux 200+

Looking for a shear flow system that seamlessly incorporates into your existing lab setup? The BioFlux 200+ offers all of the benefits of an easy-to-use and flexible system while being compatible with most inverted microscopes. It provides an affordable and complete shear flow system for the analysis of up to 24 independent samples per plate.

BioFlux 1000HT
The Completely Customizable BioFlux Solution for Ultimate Imaging Flexibility

BioFlux 1000HT

Looking for the ultimate turnkey solution for imaging flexibility and capability? The BioFlux 1000HT offers unmatched flexibility with respect to microscope options, software analysis, and image capture. It provides a high throughput platform for running physiologically relevant shear flow assays.

BioFlux 200+

The BioFlux Solution for Your Existing Microscope

The BioFlux 200+ offers all of the benefits of an easy-to-use and flexible system while being compatible with most inverted microscopes. It provides a high-content screening platform for running physiologically-relevant shear flow assays.

A shear flow system that seamlessly incorporates into your existing lab setup.

BioFlux 1000HT

The Completely Customizable BioFlux Solution for Ultimate Imaging Flexibility

The BioFlux 1000HT offers nearly unlimited flexibility with respect to microscope options, software analysis, and image capture. It provides a high content screening platform for running physiologically-relevant shear flow assays.

The ultimate turnkey solution for imaging flexibility and capability.

BioFlux Applications

Antimicrobial Screens

Analyze effectiveness of novel antimicrobials on biofilm growth under shear flow

Biofilms

Anti-biofilm drugs discovery and treatment

Cancer Research Applications

Create two adjacent micro-environments to analyze cell migration and invasion.

Cell Adhesion & Rolling

Visualize and quantify the leukocyte adhesion cascade over time using time-lapse microscopy

Host-Pathogen Interactions

Form fungal and bacterial biofilms on epithelial monolayers in vitro

Medical Device Infections

PDMS and custom microfluidic plates mimic medical device infection environment

Platelet Aggregation & Adhesion

Study the mechanisms of thrombus formation and treatment strategies under biological flow

Vascular Biology

Image properly aligned endotheial cells, examine cell permeability, and more

More Samples, Less Spend

Save Time and Money

Slide-based parallel plate flow chambers may seem like a good way to enhance the physiological relevance of your experiments; however, the setup, validation, and consumable costs can be a burden.

BioFlux Shear Flow Systems streamline physiological assays with rapid setup and zero calibration, at a fraction of typical consumable costs.

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Watch a Demonstration

Watch a live virtual demonstration of a BioFlux Shear Flow System in action.

What Customers Say

“All of these experiments probably would have taken me another 2-3 years if I had to do them in vivo or in vitro.”

“Because it’s so easy to use and we can have undergrads collecting data, it’s pretty easy to publish quickly.”

“Our lab works in a very specific field, very niche. We were looking for the right tool, with controlled shear, to fit our needs.”

“When we combine all of those needs that we had when we thought about a diagnostic tool for Sickle cell disease and we looked at the options that were available, we really like the BioFlux system because it met the pros that we thought were most meaningful in the type of test that we wanted to create.”

“I can’t go a day without using the machine."

Penn

customer U Penn

BioFlux FAQs

Can BioFlux systems be used in an aseptic environment?

Yes! BioFlux controllers measure 12.5 in (31.75 cm) width x 9.5 in (24.13 cm) height x 13 in (33.02 cm) length (not including tubing and interface), which easily fits into most laminar flow hoods. In addition, all BioFlux plates are individually sterile packaged. Furthermore, because BioFlux systems use pneumatic pressure to drive shear flow, there is no contact between the interface or tubing with anything on the BioFlux plate. This means that the BioFlux tubing remains clean and does not need to be cleaned or changed between experiments.

What type of microscope can I use with BioFlux systems?

Because BioFlux plates are Society for Biomolecular Screening (SBS) standard size, BioFlux systems are compatible with most inverted microscopes.

Do have to use an entire BioFlux plate all at once?

No, every BioFlux plate comes with a sterile adhesive sheet that can be placed on the plate as soon as it is opened. The user can then simply pierce the sheet for the wells that will be used for the experiment, saving the unused wells for later experiments.

Is analysis software included?

All BioFlux systems include standard BioFlux Control Software. Advanced analysis can be performed using BioFlux Montage Plus software, which can be purchased anytime for BioFlux 200+. BioFlux Montage Plus software is standard with BioFlux 1000HT. Furthermore, BioFlux data can easily be exported to the analysis software of your choice.

Is a plate heater included with all BioFlux systems?

All BioFlux controllers are standardly equipped with heating plate connections. However, a glass bottom heating plate (200+) or a heating adapter for the microscope stage (1000HT) must be purchased separately.

What other options are available? Can I upgrade after purchasing?

Heating options for either BioFlux 1000HT or BioFlux 200+ can easily be added at any time. BioFlux Montage Plus software is standard for BioFlux 1000HT systems and can be purchased separately for BioFlux 200+ systems. Additional features, including pulsatile flow and dual gas are now standard features of all BioFlux Shear Flow Systems.

CellRaft Technology FAQs (JetEngine)

How can I do shear flow experiments with whole blood?

Whole blood shear flow experiments can be performed using a microfluidic platform that applies controlled shear stress while supporting real-time imaging and biologically relevant surfaces. The BioFlux Shear Flow System enables whole blood perfusion through plate-based microchannels, supporting studies of adhesion, thrombus formation, and other blood-cell interactions under physiological flow conditions.

How can vascular-endothelium microenvironments be studied under shear flow conditions?

Vascular-endothelium microenvironments can be studied by culturing endothelial cells in microfluidic channels and exposing them to defined shear stress that mimics blood flow. The BioFlux Shear Flow System supports endothelial cell coatings, controlled flow profiles, and live-cell imaging, supporting researchers in modeling vessel-like environments in vitro with greater physiological relevance.

How can I do downstream genomic analyses after shear flow experiments?

Downstream genomic analyses after shear flow experiments can be done by exposing cells to controlled flow, then recovering those cells for RNA extraction, sequencing, or other molecular workflows. With the BioFlux Shear Flow System, researchers can perform shear flow studies in a reproducible microfluidic environment and then collect samples for downstream genomic analysis to connect mechanical stimulation with gene-expression changes.

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